DNA content of a functioning chicken kinetochore

• DOI: 10.1007/s10577-014-9410-3
• Publication Date: 2014-03-18T05:10:44Z
• Authors: Susana Abreu Ribeiro; Paola Vagnarelli; William C. Earnshaw
• Keywords: Protein Folding; 0303 health sciences; Chromosomal Proteins, Non-Histone; condensin; heterochromatin; kinetochores; 612; DNA; Autoantigens; Cell Line; Gene Knockout Techniques; 03 medical and health sciences; centromere; Genetics; Image Processing, Computer-Assisted; Animals; chromosome; Kinetochores; Chickens; Centromere Protein A; Original Research

In order to understand the three-dimensional structure of functional kinetochore in vertebrates, we require a complete list and stoichiometry for protein components kinetochore, which can be provided by genetic proteomic experiments. We also need know how chromatin-containing CENP-A, makes up structural foundation is folded, much that DNA involved assembling kinetochore. this MS, demonstrate functioning metaphase kinetochores chicken DT40 cells contain roughly 50 kb DNA, an amount corresponds extremely closely length chromosomal associated with CENP-A ChIP-seq Thus, during assembly, chromatin compacted into inner plate without including significant amounts flanking pericentromeric heterochromatin.