Stress responses of bivalve mollusc Unio tumidus from two areas to ibuprofen, microplastic and their mixture

• DOI: 10.1007/s10646-022-02594-8
• Publication Date: 2022-10-08T07:02:38Z
• Authors: Viktoria Martyniuk; Brigita Gylytė; Tetiana Matskiv; Vira Khoma; Halyna Tulaidan; Lesya Gnatyshyna; Kateryna Orlova-H...; Levonas Manusadži...; Oksana Stoliar
• Keywords: 0301 basic medicine; Superoxide Dismutase; Microplastics; Ibuprofen; Thiobarbituric Acid Reactive Substances; Antioxidants; 6. Clean water; Bivalvia; Oxidative Stress; 03 medical and health sciences; Unio; Animals; Plastics; Water Pollutants, Chemical

Even though bivalve molluscs are recognized as bioindicators of freshwater quality, their responses to multiple stressors are unpredictable. This study aims to elucidate the inter-population peculiarities of the effect in the sub-chronic environmentally relevant exposure to novel contaminants. The specimens of Unio tumidus from reference (Pr) and contaminated (Ct) areas were treated with ibuprofen (IBU, 0.8 µg L-1), microplastic (MP, 1.0 mg L-1, size 0.1-0.5 mm), or their combination (Mix) for 14 days. Untreated mussels (PrC- and CtC-groups) served as controls. The PrC-group had higher levels of antioxidants Mn-SOD, Cu,Zn-SOD, catalase, and cholinesterase (AChE) as well as lesser levels of oxidative lesions (TBARS and protein carbonyls) in digestive glands, indicating lower environmental impact than in the CtC-group. However, lysosomal stability was similar in both control groups. Among antioxidants, Mn-SOD activity was affected most prominently, increasing in all exposed Ct-groups. TBARS level was increased only in PrMP-group compared to responsive control. IBU and Mix enhanced protein carbonyl concentration in the Pr-groups, and decreased it in the Ct-groups. AChE was induced in the CtIBU- and PrMix-groups, and lysosomal integrity increased in the CtIBU and CtMix-groups. Discriminant analyses indicated lesser differences between Pr-groups, demonstrating lower cumulative stress compared to Ct-groups. Generally, the most remarkable response was revealed in the CtIBU-group, and distortion of individual effects was established in combined exposures. The qualification of stress-neutral and stress-positive populations was proposed for Pr- and Ct-populations correspondingly. Inter-site peculiarities must be taken into consideration when the environmental impact of MP and pharmaceuticals is evaluated.