The potential of high-resolution BAC-FISH in banana breeding

• DOI: 10.1007/s10681-008-9830-2
• Publication Date: 2008-10-18T07:42:59Z
• Authors: Guy De Capdeville; Manoel Teixeira S...; Dóra Szinay; Leandro Eugênio C...; Erik Wijnker; Rony Swennen; Gerrit Haatje Jan...; Hans De Jong
• Keywords: Introgression; Bacterial artificial chromosome; Molecular breeding

The genetic complexity in the genus Musa has been subject of study many breeding programs worldwide. Parthenocarpy, female sterility, polyploidy different cultivars and limited amount genomic information make production new banana difficult time consuming. In addition, it is known that part related wild species contain numerous chromosomal rearrangements. order to produce more effectively breeders must better understand differences potential crossing parents for introgression hybridization, but extensive lacking. As an alternative achieve on collinearity we use modern chromosome map technology as high-resolution fluorescent situ hybridization (FISH). This article presents technical aspects applications such a species. technique deals with BAC clone positioning pachytene chromosomes Calcutta 4 (Musa acuminata ssp. burmanicoides, A genome group, section Eumusa) M. velutina (section Rodochlamys). Pollen mother cells digestion pectolytic enzymes maceration acetic acid were optimized making cell spread preparations appropriate FISH. example this approach chose clones markers resistance genes hybridize them establishing their relative positions two Technical challenges adapting existing protocols are presented. We also discuss how can be instrumental validating between method helpful future mapping initiatives, allows identification rearrangements cultivars.