QTL mapping integrated with BSA-Seq analysis identifies a novel gene conferring resistance to brown planthopper from common wild rice (Oryza rufipogon Griff.)

• DOI: 10.1007/s10681-021-02964-z
• Publication Date: 2022-02-26T04:31:42Z
• Authors: Xuan Wang; Yue Han; Yue-xiong Zhang; Biao Deng; Bi-qiu Wu; Xin-ying Guo; Yu-fen Qin; Yao-yu Fang; Fang Liu; Bao-xiang Qin; Ji-jing Luo; Rong-bai Li
• Keywords: Oryza rufipogon; Brown planthopper; Germ plasm; Genetic linkage; Oryza; Marker-Assisted Selection

Abstract The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive rice pests worldwide. GXU202 is a germplasm of common wild rice (Oryza rufipogon Griff. ) with high resistance to the BPH. In this study, the genetic analysis indicated that the BPH resistant phenotype of GXU202 is controlled by a major gene. Through the combination and comparison of QTL linkage and BSA-seq analyses, a novel gene locus BPH41 conferring BPH resistance was identified, which has been finely mapped to a 114-kb region delimited by D01031 and W1 on chromosome 4. The markers D01031 and D01045 showed high accuracy in predicting resistant phenotypes to BPH, suggesting their reliability for marker-assisted selection of BPH41 in breeding for BPH resistant rice varieties. The present identification of BPH41 will establish a foundation for further map-based cloning and functional characterization of the gene.