Effects of dietary yeast inclusion and acute stress on post-prandial whole blood profiles of dorsal aorta-cannulated rainbow trout

• DOI: 10.1007/s10695-016-0297-0
• Publication Date: 2016-09-27T18:49:45Z
• Authors: David Huyben; Aleksandar Vidakovic; Andreas Nyman; Markus Langeland; Torbjörn Lundh; Anders Kiessling
• Keywords: Blood Glucose; 0301 basic medicine; Anemia, Hemolytic; Hydrocortisone; Physiology; 590; Aquatic Science; Postprandial Period; Biochemistry; Animal Feed; Article; Diet; Fish Diseases; 03 medical and health sciences; Oncorhynchus mykiss; Fish and Aquacultural Science; Acute Disease; Saccharomycetales; Animals; 14. Life underwater; Aorta; Stress, Psychological

Yeast is a potential alternative to fish meal in diets for farmed fish, yet replacing more than 50 % of results reduced growth. In 4-week experiment, 15 rainbow trout (Oncorhynchus mykiss) were cannulated and fed three each week: 30 as control (FM); 60 replacement protein, on digestible basis, with Saccharomyces cerevisiae (SC); Wickerhamomyces anomalus S. mix (WA). Blood was collected at 0, 3, 6, 12 24 h after feeding. the final week, exposed 1-min netting stressor evaluate possible diet–stress interactions. Significant increases pH, TCO2, HCO3 base excess found SC WA compared FM, which elevated blood alkaline tides. ingredients had lower buffering capacity ash content meal, explained increase addition, diet significantly erythrocyte area increased mean corpuscular haemoglobin levels, indicating haemolytic anaemia. Higher levels nucleic acid yeast-based potentially higher production reactive oxygen species suspected damaging haemoglobin, require by smaller immature erythrocytes. Acute stress caused expected rise cortisol glucose but no interaction found. These show that protein yeasts can induce anaemia trout, may limit yeast inclusion fish.