Association study between some cultivated species and their wild relatives from Apiaceae, Asteraceae and Brassicaceae families based on molecular and DNA barcoding in Egypt

• DOI: 10.1007/s10722-023-01681-x
• Publication Date: 2023-08-13T11:01:36Z
• Authors: Nerveen M. Essa; Amira A. Ibrahim; Magda I. Soliman
• Keywords: Brassicaceae; Apiaceae; Raphanus; DNA Barcoding; Capitata; Daucus carota; Cultivated plant taxonomy; Germ plasm

Abstract Concerns about food security worldwide have been raised due to the adverse impacts of climate change on agricultural productivity. Utilizing wild relatives cultivated plants in breeding schemes is a potential solution address abovementioned challenges. Wild refer indigenous plant species that share genetic ancestry with recently plants. The capacity withstand abiotic stresses and enhance crop yield stability cold arid environments renders them valuable prospective resource for programs. Molecular markers are employed assess variability their under investigation. This study set five primers derived from ISSR (Inter Simple Sequence Repeats) SCoT (Start Codon Targeted) comparative research between three families: Apiaceae ( Apium gravolens L., A. leptophyllum (Pers.) F.Muell., Daucus carota L . & D. litoralis Sm.), Asteraceae Lactuca sativa L. serriola L.) Brassicaceae B. oleracea Brassica tourneforttii Gouan., Raphanus sativus R. raphanistrum L.). technique yields approximately 206 bands, whereas generate 207 bands. yielded 169 polymorphic exhibiting polymorphism rate 82.04%, produced bands 81.64%. has found exhibit highest resolving power (RP) 48.25, as determined by ISSR. primary objective DNA barcoding create communal database sequences can be utilized identify organisms clarify taxonomic classification. findings indicate internal transcribed spacer (ITS) regions high efficacy tool. Overall, results obtained two molecular analyses suggest significant degree diversity relatedness among taxa examined this study.