Effect of galactofucan sulfate of a brown seaweed on induced hepatotoxicity in rats, sodium pentobarbital-induced sleep, and anti-inflammatory activity

• DOI: 10.1007/s10811-015-0698-y
• Publication Date: 2015-09-23T02:05:24Z
• Authors: Luiza Sheyla Even...; Allisson J. Gomes...; Marília da S. Nas...; Thuane de Sousa P...; Kahena de Quevedo...; Luciana G. Alves; Eliane Marinho So...; Renata Mendonça A...; Edda Lisboa Leite
• Keywords: Hepatoprotection

This study was designed to characterize the chemical composition, anti-oxidant activity, anti-inflammatory, and hepatoprotective effect of the polysaccharide a galactofucan sulfate (GFS) from brown seaweed Lobophora variegata. Chemical characterization was performed by several chemical methods, high-pressure liquid chromatography (HPLC), nuclear magnetic resonance (NMR) (1H, 13C, heteronuclear multiple quantum correlation (HMQC), and 1H-1H COSY), and infrared spectroscopies. The results showed an acidic polysaccharide with a high level of sulfated fucose and galactose. GFS displayed anti-oxidant effects acting directly on the free radicals. Pretreatment with fucans in mice induced by carbon tetrachloride (CCl4) administration resulted in a significantly inhibited increase in the blood enzymatic activities and bilirubin level. Administration of GFS (75 mg kg−1) reduced (p < 0.01) the activities of enzymes. This was shown by assessment of hepatic markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamil transferase (γ-GT), and bilirubin reduction in serum. Histological analysis of the liver confirmed the effects of fucans on hepatoprotection. This fucan affects time of sodium pentobarbital-induced sleep, also demonstrating its effect on hepatic recuperation.