Comparison of mesenchymal stem cell proliferation and differentiation between biomimetic and electrochemical coatings on different topographic surfaces

• DOI: 10.1007/s10856-012-4789-x
• Publication Date: 2012-10-09T08:07:43Z
• Authors: Elena García-Gareta; Jia Hua; Jonathan C. Knowles; Gordon W. Blunn
• Keywords: Calcium Phosphates; 0301 basic medicine; Sheep; Cell Differentiation; Mesenchymal Stem Cells; DNA; Electrochemical Techniques; Alkaline Phosphatase; 03 medical and health sciences; Coated Materials, Biocompatible; Biomimetics; Microscopy, Electron, Scanning; Animals; Cell Proliferation

The hypothesis for this study was that there is no difference in mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation between calcium-phosphate (CaP) coatings with different crystal size deposited on different topographic surfaces of metal discs. Polished (P) and sand-blasted (SB) tantalum and TiAl6V4 discs were CaP coated by three methods-biomimetic (BioM), electrochemical at 20 mA/cm(2) and at 6.5 mA/cm(2)-and cultured with MSCs. At days 4, 7 and 14, cell proliferation-alamarBlue(®) activity and DNA quantification-and differentiation down the osteogenic lineage-ALP activity normalised per amount of DNA and SEM (morphology)-were analysed. Results showed that MSCs proliferated more when cultured on the nano-sized BioM coatings compared to uncoated and electrochemically coated discs. MSCs also proliferated more on P surfaces than on SB and or electrochemical coatings. All the coatings induced osteogenic differentiation, which was greater on electrochemical coatings and SB discs.