Biofunctionalization strategies on tantalum-based materials for osseointegrative applications

• DOI: 10.1007/s10856-015-5445-z
• Publication Date: 2015-02-10T05:53:29Z
• Authors: Carlos Mas-Moruno; Beatriz Garrido; Daniel Rodriguez; Elisa Ruperez; F. Javier Gil
• Keywords: Silanization; Biocompatibility; Bioconjugation; Surface Modification; Protein Adsorption; Biomaterial; Hybrid material; Biointerface

The use of tantalum as biomaterial for orthopedic applications is gaining considerable attention in the clinical practice because it presents an excellent chemical stability, body fluid resistance, biocompatibility, and more osteoconductive than titanium or cobalt-chromium alloys. Nonetheless, metallic biomaterials are commonly bioinert may not provide fast long-lasting interactions with surrounding tissues. short cell adhesive peptides derived from extracellular matrix has shown to improve adhesion accelerate implant's biointegration vivo. However, this strategy been rarely applied materials. In work, we have studied two immobilization strategies (physical adsorption covalent binding via silanization) functionalize surfaces a RGD peptide. Surfaces were used untreated activated either HNO3 UV/ozone treatments. process biofunctionalization was characterized by means physicochemical biological methods. Physisorption peptide on control HNO3-treated significantly enhanced attachment spreading osteoblast-like cells; however, no effect observed ozone-treated samples. This attributed inefficient these highly hydrophilic surfaces, evidenced contact angle measurements X-ray photoelectron spectroscopy. contrast, activation proved be most efficient method support silanization subsequent attachment, displaying highest values adhesion. study demonstrates that both physical feasible methods immobilize onto tantalum-based materials, providing them superior bioactivity.