Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

• DOI: 10.1007/s10856-021-06630-7
• Publication Date: 2022-01-20T11:05:36Z
• Authors: Ying Liu; Yunen Liu; Mi Wu; Rufei Zou; Shun Mao; Peifang Cong; Mingxiao Hou; Hongxu Jin; Yan Zhao; Yongli Bao
• Keywords: Wound Healing; 0303 health sciences; Tissue Scaffolds; Nanofibers; Biocompatible Materials; Chitin; Hydrogels; Mesenchymal Stem Cells; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; TA401-492; Medical technology; Adipocytes; Animals; R855-855.5; Materials of engineering and construction. Mechanics of materials; Original Research

Because of stem cells are limited by the low efficiency their cell homing and survival in vivo, delivery systems scaffolds have attracted a great deal attention for cells' successful clinical practice. β-chitin nanofibers (β-ChNF) were prepared from squid pens this study. Fourier transform infrared spectroscopy, X-ray diffraction scanning electron microscopy proved that β-ChNFs with diameter 5 to 10 nm prepared. β-ChNF dispersion became gelled upon addition culture medium. Cell experiments showed exhibited negligible cytotoxicity towards ADSCs L929 cells, it was found more exosomes secreted globular grown hydrogel. The vivo rats ADSCs-loaded hydrogel could directly cover wound surface significantly accelerate healing promote generation epithelization, granulation tissue collagen. In addition, clearly regulated expressions VEGFR, α-SMA, collagen I III. Finally, we activated TGFβ/smad signaling. neutralization TGFβ markedly reduced Smad phosphorylation TIMP1, VEGFR α-SMA. Taken together, these findings suggest promises treating wounds challenge heal via conventional methods. Graphical abstract.