Exclusively NOESY-based automated NMR assignment and structure determination of proteins

• DOI: 10.1007/s10858-011-9502-8
• Publication Date: 2011-03-29T11:52:59Z
• Authors: Teppei Ikeya; Jun-Goo Jee; Yoshiki Shigemitsu; Junpei Hamatsu; Masaki Mishima; Yutaka Ito; Masatsune Kainosho; Peter Güntert
• Keywords: Nuclear Overhauser effect

A fully automated method is presented for determining NMR solution structures of proteins using exclusively NOESY spectra as input, obviating the need to measure any spectra only for obtaining resonance assignments but devoid of structural information. Applied to two small proteins, the approach yielded structures that coincided closely with conventionally determined structures.