UHPLC-ESI/TOFMS Determination of Salicylate-like Phenolic Gycosides in Populus tremula Leaves

• DOI: 10.1007/s10886-011-9991-7
• Publication Date: 2011-07-05T09:23:46Z
• Authors: Ilka Nacif Abreu; Maria Ahnlund; Thomas Moritz; Benedicte Riber A...
• Keywords: Salicin; Chemotype; Arbutin

Associations of salicylate-like phenolic glycosides (PGs) with biological activity have been reported in Salix and Populus trees, but only for a few compounds, relation to limited number herbivores. By considering the full diversity PGs, we may improve our ability recognize genotypes or chemotype groups enhance understanding their ecological function. Here, present fast efficient general method salicylate determination leaves Eurasian aspen that uses ultra-high performance liquid chromatography-electrospray ionization/time-of-flight mass spectrometry (UHPLC-ESI/TOFMS). The time required chromatography separations was 13.5 min per sample, compared around 60 sample most HPLC protocols. In leaf samples from identical P. tremula diverse propagation treatment histories, identified nine PGs. We found compound-specific chromatograms be more informative than UV-visible compound identification when quantitating large variability PG content. Signature compounds previously tremoloides (tremulacin, tremuloidin, salicin, salicortin) always were present, five PGs (2'-O-cinnamoyl-salicortin, 2'-O-acetyl-salicortin, 2'-O-acetyl-salicin, acetyl-tremulacin, salicyloyl-salicin) detected first tremula. using information about formic acid adduct appeared LTQ-Orbitrap MS environment, novel like acetyl-tremulacin could tentatively without use standards. consistently either regardless damage not detectable. some genotypes, concentrations 2'-O-acetyl-salicortin 2'-O-cinnamoyl-salicortin similar levels biologically active other Salicaceous trees. Our study suggests expect wide variation content populations which is interest both studies interactions herbivores mapping population structure.