Fungal Alkaloid Occurrence in Endophyte-Infected Perennial Ryegrass during Seedling Establishment

• DOI: 10.1007/s10886-020-01162-w
• Publication Date: 2020-03-03T11:03:10Z
• Authors: Katrin G. Hewitt; Wade J. Mace; Catherine M. McKe...; Cory Matthew; Alison J. Popay
• Keywords: 0301 basic medicine; 2. Zero hunger; 03 medical and health sciences; Alkaloids; Seedlings; Epichloe; Endophytes; Lolium; 15. Life on land; Symbiosis

The symbiotic Epichloë festucae var. lolii endophyte produces alkaloids which can provide its host grass, perennial ryegrass (Lolium perenne L), with a selective advantage in both natural and agricultural managed ecosystems. This study focuses on understanding the alkaloid concentrations that occur in endophyte-infected perennial ryegrass during the early establishment phase. In a glasshouse experiment fungal alkaloid concentrations (peramine, lolitrem B, ergovaline, and epoxy-janthitrems) were measured in perennial ryegrass seedlings infected with E. festucae var. lolii proprietary strains AR1, AR37, NEA2, and NZ common toxic for 69 days after sowing. The endophyte becomes metabolically active, starting alkaloid production, as early as 6 days after sowing. Alkaloid concentrations peaked in 8- to 10- day-old seedlings due to a seedling growth slowdown. This study provides data showing that the loss of insect protection in endophyte-infected seedlings is linked to a reduction in chemical defence after seed-stored, maternally synthesised alkaloids are diluted by seedling dry matter accumulation.