A Rapid Method to Measure Serum Retinol Concentrations in Japanese Black Cattle Using Multidimensional Fluorescence

• DOI: 10.1007/s10895-020-02640-w
• Publication Date: 2020-10-22T21:02:17Z
• Authors: Yoshio Tamura; Hiroki Inoue; Satoshi Takemoto; Kazuo Hirano; Kazutoshi Miyaura
• Keywords: 0403 veterinary science; 2. Zero hunger; Spectrometry, Fluorescence; Animals; Original Article; Cattle; 04 agricultural and veterinary sciences; Vitamin A; Fluorescence; Chromatography, High Pressure Liquid

Abstract Vitamin A levels in fattening Japanese Black cattle affect meat quality; therefore, it is important to monitor serum retinol concentrations. To simplify and accelerate the evaluation of concentrations cattle, we developed a new predictive method using excitation-emission matrix (EEM) fluorescence spectrophotometry. For analytical comparison, concentration was also measured conventional HPLC method. We examined excitation (Ex) emission (Em) wavelengths serum, which were 250–450 250–600 nm, respectively. Parallel factor analysis separated four components from EEM data, one related retinol. Next, partial least square regression model created obtained EEMs as explanatory variables accrual measurement values objective variables. The determination coefficient value ( R 2 ), root mean squared error prediction (RMSEP), ratio performance deviation (RPD) determined. comparison with reference found that , RMSEP, RPD calibration 0.95, 6.4 IU/dl, 4.2, This implies can estimate high accuracy. Additionally, fluorescent peaks contributed calibration, extracted variable importance projection plots, Ex/Em = 320/390 330/520 nm. Thus, assume this observes not only free retinol, but retinol-binding protein. In conclusion, multidimensional accurately quickly determine cattle.