Radiosynthesis and in vivo evaluation of [125I]2-(4-iodophenethyl)-2-methylmalonic acid as a potential radiotracer for detection of apoptosis

• DOI: 10.1007/s10967-015-4346-4
• Publication Date: 2015-08-11T01:26:58Z
• Authors: Jongho Jeon; Ha Eun Shim; Sajid Mushtaq; Jung Ae Kang; You Ree Nam; Seonhye Yoon; Hye Rim Kim; Dae Seong Choi; Beom Su Jang; Sang Hyun Park
• Keywords: Radiosynthesis; Biodistribution; PET Imaging

The aim of this study is to synthesize 125I-labeled 2-(4-iodophenethyl)-2-methylmalonic acid ([125I]IMA) for the development of new apoptosis imaging tracer. The optimized radiolabeling procedure provided [125I]IMA with high radiochemical yield (75.5 ± 5.2 %) and radiochemical purity (>99 %) within 100 min. Specific radioactivity of [125I]IMA was 31.0 MBq/μmol. Biodistribution study of [125I]IMA was carried out using ICR mouse and the result showed the uptake values in apoptotic cells of the testes of the male mice were 1.7–3.2 fold higher than those of leg muscle. Therefore, [125I]IMA will be a promising radiotracer for in vivo SPECT imaging of apoptotic cells.