Mandibular muscle troponin of the Florida carpenter ant Camponotus floridanus: extending our insights into invertebrate Ca2+ regulation

• DOI: 10.1007/s10974-021-09606-w
• Publication Date: 2021-07-13T15:03:24Z
• Authors: Yun Shi; Julia P. Bethea; Hannah L. Hetzel-...; Maicon Landim-Vieira; Ross J. Mayper; Regan L. Williams; Lauren E. Kessler; Amanda M. Ruiz; Kathryn Gargiulo; Jennifer S. M. Rose; Grayson Platt; Jose R. Pinto; Brian K. Washburn; P. Bryant Chase
• Keywords: 0301 basic medicine; 0303 health sciences; Ants; Mandible; Invertebrates; 03 medical and health sciences; Troponin T; Animals; Humans; Calcium; Muscle, Skeletal; Troponin C

Ants use their mandibles for a variety of functions and behaviors. We investigated mandibular muscle structure and function from major workers of the Florida carpenter ant Camponotus floridanus: force-pCa relation and velocity of unloaded shortening of single, permeabilized fibres, primary sequences of troponin subunits (TnC, TnI and TnT) from a mandibular muscle cDNA library, and muscle fibre ultrastructure. From the mechanical measurements, we found Ca2+-sensitivity of isometric force was markedly shifted rightward compared with vertebrate striated muscle. From the troponin sequence results, we identified features that could explain the rightward shift of Ca2+-activation: the N-helix of TnC is effectively absent and three of the four EF-hands of TnC (sites I, II and III) do not adhere to canonical sequence rules for divalent cation binding; two alternatively spliced isoforms of TnI were identified with the alternatively spliced exon occurring in the region of the IT-arm α-helical coiled-coil, and the N-terminal extension of TnI may be involved in modulation of regulation, as in mammalian cardiac muscle; and TnT has a Glu-rich C-terminus. In addition, a structural homology model was built of C. floridanus troponin on the thin filament. From analysis of electron micrographs, we found thick filaments are almost as long as the 6.8 μm sarcomeres, have diameter of ~ 16 nm, and typical center-to-center spacing of ~ 46 nm. These results have implications for the mechanisms by which mandibular muscle fibres perform such a variety of functions, and how the structure of the troponin complex aids in these tasks.