Robustness of resistance surface optimisations: sampling schemes and genetic distance metrics affect inferences in landscape genetics

• DOI: 10.1007/s10980-023-01752-5
• Publication Date: 2023-08-18T10:14:20Z
• Authors: Anna Schleimer; Amanda Luttringer; Julian Wittische; Frank Drygala; Roland Proess; Lisette Cantú-Sal...; Alain C. Frantz
• Keywords: 0106 biological sciences; 15. Life on land; 01 natural sciences

AbstractContextLandscape genetics provides powerful tools to quantify the effects of landscape features on population connectivity, but robust results are imperative to inform conservation planning.ObjectivesThe robustness of landscape genetic inferences was assessed using the case of the northern crested newt (Triturus cristatus) in Luxembourg. Specifically, the effect of different study designs and genetic distance metrics was tested in terms of model convergence and misspecification rates (Type I error).MethodsThe optimisation of resistance surfaces was performed in ResistanceGA, using individual- and population-based sampling designs and 16 genetic distance metrics inferred from 897 multilocus genotypes from 85 locations. Empirical results were complemented with simulations to assess Type I error rates and correlation between ‘true’ and optimised resistance surfaces.ResultsIndividual-based optimisations seemed prone to overfitting, with little convergence among empirical resistance surfaces from different sets of individuals. Simulations showed significant differences in performance among population genetic distance metrics. Linear topographical features exhibited higher Type I error rates (83.3%) than continuous features (44.9%), suggesting potential underestimation of road-induced fragmentation effects. Jost’sD,$${F}_{ST}$$FST, and PCA axes 1–45 were the top three genetic distance metrics for recovering true resistance features. Topographic roughness consistently drove spatial genetic clustering ofT. cristatus,but variability existed among conductivity maps derived from optimised resistance surfaces.ConclusionsThese findings underscore the importance of carefully selecting genetic distance metrics and addressing potential sources of uncertainty in resistance surface optimisation. By doing so, we can enhance the effectiveness of conservation planning efforts forT. cristatusand species with similar ecological considerations.