Cloning and Expression of Oil Palm (Elaeis guineensis Jacq.) Type 2 Ribosome Inactivating Protein in Escherichia coli

• DOI: 10.1007/s10989-015-9485-5
• Publication Date: 2015-08-12T04:05:29Z
• Authors: Yung-Chie Tan; Wan-Yong Ho; Noorjahan Banu Al...; Mui-Yun Wong; Chai-Ling Ho
• Keywords: 0301 basic medicine; 03 medical and health sciences; 3. Good health

EgT2RIP is a type 2 ribosome-inactivating protein isolated from oil palm (Elaeis guineensis Jacq.). Its transcript abundance was reported to be up-regulated in oil palm roots upon inoculation of pathogenic fungus Ganoderma boninense in a recent study. This study aims to produce an active recombinant EgT2RIP protein for biological studies. The DNA fragments encoding Chain A (CA) and Chain B (CB) of EgT2RIP were cloned individually in an expression vector. Soluble CA and partially soluble CB were expressed in Escherichia coli Rosetta-gami 2 (DE3). Purified recombinant CA and CB were associated in a cysteine/cystine reduced/oxidized system, yielding a heterodimer protein (AB). The AB protein showed growth inhibitory activity against breast cancer cell lines (MCF-7) as well as non-tumorigenic breast epithelial cell line (MCF-10A) at IC50 = 1.4 and 10.9 μg mL−1, respectively. The active protein produced from this study may have the potential to be used for treatment in medical and agricultural fields.