Exercise training prevents ecto-nucleotidases alterations in platelets of hypertensive rats

• DOI: 10.1007/s11010-012-1431-7
• Publication Date: 2012-08-22T18:49:24Z
• Authors: Andréia Machado C...; Margarete Dulce B...; Caroline Curry Ma...; Fátima Hussein Ab...; Daniela Zanini; Roberta Schmatz; Jessié Gutierres; Victor Camera Pim...; Gustavo Thomé; Claudio Alberto M...; Juliano Marchi Vi...; Naiara Stefanello; Fernando da Silva...; Jucimara Baldissa...; Luiz Fernando Fre...; Adriane Bello Klein; Vera Maria Morsch; Maria Rosa Chitol...
• Keywords: Blood Platelets; Male; 0301 basic medicine; Platelet Aggregation; Adenosine Deaminase; Platelet Count; Hydrolysis; Nitric Oxide; Rats; Adenosine Diphosphate; 03 medical and health sciences; Adenosine Triphosphate; NG-Nitroarginine Methyl Ester; Physical Conditioning, Animal; Hypertension; Animals; Rats, Wistar; 5'-Nucleotidase

In this study, we investigated the effect of 6 weeks of swimming training on the ecto-nucleotidase activities and platelet aggregation from rats that developed hypertension in response to oral administration of L-NAME. The rats were divided into four groups: control (n = 10), exercise (n = 10), L-NAME (n = 10), and exercise L-NAME (n = 10). The animals were trained five times per week in an adapted swimming system for 60 min with a gradual increase of the workload up to 5 % of animal's body weight. The results showed an increase in ATP, ADP, AMP, and adenosine hydrolysis, indicating an augment in NTPDase (from 35.3 ± 8.1 to 53.0 ± 15.1 nmol Pi/min/mg protein for ATP; and from 21.7 ± 7.0 to 46.4 ± 15.6 nmol Pi/min/mg protein for ADP as substrate), ecto-5'-nucleotidase (from 8.0 ± 5.7 to 28.1 ± 6.9 nmol Pi/min/mg protein), and ADA (from 0.8 ± 0.5 to 3.9 ± 0.8 U/L) activities in platelets from L-NAME-treated rats when compared to other groups (p < 0.05). A significant augment on platelet aggregation in L-NAME group was also observed. Exercise training was efficient in preventing these alterations in the exercise L-NAME group, besides showing a significant hypotensive effect. In conclusion, our results clearly indicated a protector action of moderate intensity exercise on nucleotides and nucleoside hydrolysis and on platelet aggregation, which highlights the exercise training effect to avoid hypertension complications related to ecto-nucleotidase activities.