Beneficial effect of ER stress preconditioning in protection against FFA-induced adipocyte inflammation via XBP1 in 3T3-L1 adipocytes

• DOI: 10.1007/s11010-019-03627-3
• Publication Date: 2019-10-19T15:39:07Z
• Authors: Min Wang; Xi Chen; Zhenda Zheng; Shujie Yu; Bin Zhou; Yong Liu; Dinghui Liu; Yanming Chen; Xiaoxian Qian
• Keywords: XBP1; Tunicamycin

Abstract Adipose tissue inflammation is closely associated with the development of obesity and insulin resistance. Free fatty acids (FFAs) are a major inducer obesity-related Previously, we reported that endoplasmic reticulum (ER) stress potentially mediated retinal in diabetic retinopathy. The unfolded protein response (UPR) protects cells against damage induced by oxidative stress. X-box binding 1 (XBP1) plays role protecting modulating UPR. However, link between ER adipocyte has been poorly investigated. In present study, found pretreatment 3T3-L1 adipocytes low dose tunicamycin inhibited FFA-induced upregulated expression inflammatory cytokines. addition, FFAs phosphorylation p65 subunit NF-κB was largely adipocytes. Knockdown XBP1 siRNA markedly mitigated protective effects preconditioning inflammation. Conversely, overexpression alleviated IκB-α, IKKα/β, NF-κB, which accompanied decreased cytokine expression. Collectively, these results imply beneficial inflammation, likely through inhibition IKK/NF-κB pathway via XBP1.