Exosomes derived from human umbilical cord mesenchymal stem cells decrease neuroinflammation and facilitate the restoration of nerve function in rats suffering from intracerebral hemorrhage

• DOI: 10.1007/s11010-024-04954-w
• Publication Date: 2024-03-08T20:01:36Z
• Authors: Chengrui Nan; Yan Zhang; Aobo Zhang; Yunpeng Shi; Dongdong Yan; Zhimin Sun; Qianxu Jin; Haoran Huo; Yayu Zhuo; Zongmao Zhao
• Keywords: Ex vivo; Neuroregeneration

Abstract Exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSC-ex) have become a hopeful substitute for whole-cell therapy due to their minimal immunogenicity and tumorigenicity. The present study aimed investigate the hypothesis that hUCMSC-ex can alleviate excessive inflammation resulting intracerebral hemorrhage (ICH) facilitate rehabilitation of nervous system in rats. In vivo, hemorrhagic stroke was induced by injecting collagenase IV into striatum rats using stereotactic techniques. were injected via tail vein at 6 h after ICH model establishment dosage 200 µg. vitro, astrocytes pretreated with then stimulated hemin (20 μmol/mL) establish an cell model. expression TLR4/NF-κB signaling pathway proteins inflammatory factors, including TNF-α, IL-1β, IL-10, assessed both vivo vitro impact on inflammation. neurological function evaluated corner turn test, forelimb placement Longa score, Bederson score 1st, 3rd, 5th day. Additionally, RT-PCR employed examine mRNA TLR4 following treatment. findings demonstrated downregulated protein TLR4, NF-κB/P65, p-P65, reduced levels pro-inflammatory cytokines TNF-α increased anti-inflammatory cytokine IL-10. Ultimately, administration improved behavioral performance However, results PT-PCR indicated did not affect ICH, suggesting hUCMSCs-ex may inhibit translation rather than transcription, thereby suppressing pathway. We conclude mitigates hyperinflammation inhibiting This provides preclinical evidence potential future application treatment cerebral injury.