Chemically induced acute model of sarcosinemia in wistar rats

• DOI: 10.1007/s11011-015-9759-9
• Publication Date: 2015-11-11T23:33:56Z
• Authors: Rodrigo Binkowski...; Tanise Gemelli; Denise Bertin Rojas; Carlos Severo Dut...; Clovis Milton Duv...
• Keywords: Sarcosine; Pathophysiology; Animal model

In the present study, we developed an acute chemically induced model of sarcosinemia in Wistar rats. Wistar rats of 7, 14 and 21 postpartum days received sarcosine intraperitoneally in doses of 0.5 mmol/Kg of body weight three time a day at intervals of 3 h. Control animals received saline solution (NaCl 0.85 g%) in the same volume (10 mL/Kg of body weight). The animals were killed after 30 min, 1, 2, 3 or 6 h after the last injection and the brain and the blood were collected for sarcosine measurement. The results showed that plasma and brain sarcosine concentrations achieved levels three to four times higher than the normal levels and decreased in a time-dependent way, achieving normal levels after 6 hours. Considering that experimental animal models are useful to investigate the pathophysiology of human disorders, our model of sarcosinemia may be useful for the research of the mechanisms of neurological dysfunction caused by high tissue sarcosine levels.