Prolongation of seed viability and grain quality in rice by editing OsLOX1 using CRISPR/Cas9

• DOI: 10.1007/s11032-024-01506-4
• Publication Date: 2024-10-12T05:02:05Z
• Authors: Changling Mou; Yaping Chen; Ping Zhang; Qikai Tong; Ziyan Zhu; Tengfei Ma; Ping Wang; Kai Fu; Cheng Chen; Yunshuai Huang; Fulin Zhang; Qixian Hao; Min Zhang; Shijia Liu; Ling Jiang; Jianmin Wan
• Keywords: Grain Quality

Deterioration of rice (Oryza sativa L.) affects grain quality and seed viability during storage. Lipoxygenase (LOX), a key enzyme in lipid metabolism, directly the rate ageing. Here, we found that knock-out lipoxygenase gene OsLOX1 by CRISPR/Cas9 delayed loss quality. Transcriptome analysis showed storage, affected transcription multiple genes, including genes related to metabolism antioxidant pathways such as phosphatase acetaldehyde dehydrogenase, which may regulate storability. The significantly down- up-regulated only Ningjing 4 after NA for 13 months 3 days AA suggesting likely promoted balancing ageing storage regulated storability through amino acid synthesis metabolic pathways. Moreover, without not improved viability, but also had little impact on agronomic traits. More importantly, lines were approved 2019 (Agricultural Foundation China Report No. 770). Collectively, our study is beneficial prolongation can be applied agricultural production.