Evaluation of cytochrome b mtDNA sequences in genetic diversity studies of Channa marulius (Channidae: Perciformes)

• DOI: 10.1007/s11033-010-0175-2
• Publication Date: 2010-05-04T07:18:57Z
• Authors: Maria Habib; W. S. Lakra; Vindhya Mohindra; Praveen Khare; A. S. Barman; Akanksha Singh; Kuldeep K. Lal; Peyush Punia; Asif A. Khan
• Keywords: 0303 health sciences; Polymorphism, Genetic; Geography; Genetic Variation; Sequence Analysis, DNA; Cytochromes b; DNA, Mitochondrial; Mitochondria; Perciformes; 03 medical and health sciences; Genetics, Population; Haplotypes; Animals; 14. Life underwater

Channa marulius (Hamilton, 1822) is a commercially important freshwater fish and a potential candidate species for aquaculture. The present study evaluated partial Cytochrome b gene sequence of mtDNA for determining the genetic variation in wild populations of C. marulius. Genomic DNA extracted from C. marulius samples (n = 23) belonging to 3 distant rivers; Mahanadi, Teesta and Yamuna was analyzed. Sequencing of 307 bp Cytochrome b mtDNA fragment revealed the presence of 5 haplotypes with haplotype diversity value of 0.763 and nucleotide diversity value of 0.0128. Single population specific haplotype was observed in Mahanadi and Yamuna samples and 3 haplotypes in Teesta samples. The analysis of data demonstrated the suitability of partial Cytochrome b sequence in determining the genetic diversity in C. marulius population.