Luteolin inhibits migration of human glioblastoma U-87 MG and T98G cells through downregulation of Cdc42 expression and PI3K/AKT activity

• DOI: 10.1007/s11033-013-2632-1
• Publication Date: 2013-05-15T10:21:35Z
• Authors: Wen-Yu Cheng; Ming-Tsang Chiao; Yea-Jiuen Liang; Yi-Chin Yang; Chiung-Chyi Shen; Chiou-Ying Yang
• Keywords: CDC42; MG132

Luteolin (3',4',5,7-tetrahydroxyflavone) is a common flavonoid in many types of plants and has several beneficial biological effects, including anti-inflammation, anti-oxidant, anti-cancer properties. However, the detail mechanisms luteolin suppressing tumor invasion metastasis are poorly understood. Here, we investigated effects on glioblastoma cell migration activity. Under non-cytotoxic doses (15 30 μM), exhibited an inhibitory effect U-87 MG T98G cells. Additionally, filopodia assembly cells was markedly suppressed after treatment. The treatment also showed decrease Cdc42 (cell division cycle 42) protein levels reduced PI3K/AKT activation, whereas there no association between this phosphorylated ERK or altered transcription Cdc42. Over expression constitutive (Q61L) using transient transfection induced partial migration, but did not affected degradation Moreover, inhibition proteaosome pathway by MG132 caused significant recovery ability augmented treatment, suggesting that pharmacological via likely to preferentially facilitate Taken together, study demonstrated flavonoids prevent affecting modulating facilitating their pathway.