The genetic contribution of CIDEA polymorphisms, haplotypes and loci interaction to obesity in a Han Chinese population

• DOI: 10.1007/s11033-013-2671-7
• Publication Date: 2013-09-20T06:34:34Z
• Authors: Jingjing Wu; Ling Zhang; Jie Zhang; Ying Dai; Lili Bian; Manshu Song; Alyce Russell; Wei Wang
• Keywords: Male; Multifactor Dimensionality Reduction; genetic association; [RSTDPub]; single nucleotide polymorphism; Models; genetic variability; Haplotype; Medicine and Health Sciences; Ethnicity; 2. Zero hunger; 0303 health sciences; allele; article; risk assessment; Single Nucleotide; matrix assisted laser desorption ionization time of flight mass spectrometry; Middle Aged; 3. Good health; Association study; female; risk factor; Female; Asian Continental Ancestry Group; gene locus; Polymorphismadult; Ethnic Groups; Cidea gene; Polymorphism, Single Nucleotide; CIDEA; 03 medical and health sciences; male; Genetic; Asian People; 616; Humans; controlled study; Genetic Predisposition to Disease; human; Obesity; Polymorphism; gene; Genetic Association Studies; gene location; Chinese; Models, Genetic; gene interaction; case control study; major clinical study; Logistic Models; Haplotypes; Genetic Loci; Case-Control Studies; Apoptosis Regulatory Proteins

To investigate the association of tag-SNPs and haplotype structures of the CIDEA gene with obesity in a Han Chinese population. Five single nucleotide polymorphisms (SNPs) (rs1154588/V115F, rs4796955/SNP1, rs8092502/SNP2, rs12962340/SNP3 and rs7230480/SNP4) in the CIDEA gene were genotyped in a case-control study. Genotyping was performed using the sequenom matrix-assisted laser desorption/ionization time-of-flight mass spectrometry iPLEX platform. There were significant differences between the obese and control groups in genotype distributions of V115F (P < 0.001), SNP1 (P = 0.006) and SNP2 (P = 0.005). Carriers of V115F-TT, SNP1-GG and SNP2-CC genotypes had a 2.84-fold (95 % CI 1.73-4.66), 2.19-fold (95 % CI 1.09-4.38) and 4.37-fold (95 % CI 1.21-15.08) increased risk for obesity, respectively. Haplotype analysis showed that GTTC (SNP1/SNP2/V115F/SNP4) had 1.41-fold (95 % CI 1.02-1.95) increased risk for obesity; whereas, haplotype TTGC had 0.48-fold (95 % CI 0.24-0.96) decreased risk for obesity. Using the multifactor dimensionality reduction method, the best model including SNP1, SNP2, V115F and SNP4 polymorphisms was identified with a maximum testing accuracy to 59.32 % and a perfect cross-validation consistency of 10/10 (P = 0.011). Logistic analysis indicated that there was a significant interaction between SNP1 and V115F associated with obesity. Subjects having both genotypes of SNP1/GG and V115F/TT were more susceptible to obesity in the Han Chinese population (OR 2.66, 95 %: 1.22-5.80). Genotypes of V115F/TT, SNP1/GG and SNP2/CC and haplotype GTTC of CIDEA gene were identified as risk factors for obesity in the Han Chinese population. The interaction between SNP1 and V115F could play a joint role in the development of obesity.