Association of microRNAs and pathologic response to preoperative chemotherapy in triple negative breast cancer: preliminary report

• DOI: 10.1007/s11033-014-3140-7
• Publication Date: 2014-01-28T01:22:05Z
• Authors: Agnieszka Kolacinska; Jan Morawiec; Wojciech Fendler; Beata Malachowska; Zbigniew Morawiec; Janusz Szemraj; Zofia Pawlowska; Dipanjan Chowdhury; Young Eun Choi; Robert Kubiak; Lukasz Pakula; Izabela Zawlik
• Keywords: Triple-negative breast cancer; Surgical oncology

Triple negative breast cancer (TNBC) has caught the attention of oncologists worldwide because poor prognosis and paucity targeted therapies. Gene pathways have been widely studied, but less is known about epigenetic factors such as microRNAs (miRNAs) their role in tailoring an individual systemic surgical approach for patients. The aim study was to examine selected miRNAs TNBC core biopsies sampled before preoperative chemotherapy subsequent pathologic response mastectomy or conservation specimens. Prior treatment, needle were collected from 11 female patients with inoperable locally advanced large resectable tumors suitable down-staging. In all we analyzed 19 per sample: 512, 190, 200, 346, 148, 449, 203, 577, 93, 126, 423, 129, 193, 182, 136, 135, 191, 122 222 (miRCURY LNA™ Universal RT microRNA polymerase chain reaction Custom Pick & Mixpanels). Wilcoxon signed-rank test used compare related samples. Ingenuity pathway analysis evaluate potential functional significance differentially expressed miRNAs. Statistical showed that 3 differed relation i.e. good versus poor. These differences failed reach statistical significance, although a trend observed (p = 0.06). Among these miRNAs, identified—miR-200b-3p, miR-190a miR-512-5p. summary, our results indicate higher miR-200b-3p, lower miR-512-5p expression levels may be associated better increased feasibility conserving surgery Although small cohort, they provide important basis larger, prospective, multicenter studies investigate neoadjuvant setting.