Upregulation of the clpB gene in response to heat shock and beta-lactam antibiotics in Acinetobacter baumannii

• DOI: 10.1007/s11033-019-05209-4
• Publication Date: 2019-12-02T10:37:30Z
• Authors: Waleska Yana Laza...; Elaine Luzia dos ...; José Luis da-Conc...; Marina Kimiko Kad...; Rinaldo Ferreira ...; Alexandre Maller; Rita de Cássia Ga...
• Keywords: Acinetobacter baumannii; 0301 basic medicine; 03 medical and health sciences; Bacterial Proteins; Genes, Bacterial; Gene Expression Regulation, Bacterial; beta-Lactams; Heat-Shock Response; Anti-Bacterial Agents; Up-Regulation

The role of the clpB gene encoding HSP/chaperone ClpB was evaluated in the multiresistant antibiotic cells of Acinetobacter baumannii (RS4 strain) under stress-induced heat shock and different beta-lactams. The expression of the clpB gene was assessed by qPCR during heat shock at 45 °C and subinhibitory concentrations of ampicillin (30 μg mL-1), amoxicillin + sulbactam (8/12 μg mL-1), cefepime (30 μg mL-1), sulfamethoxazole + trimethoprim (120/8 μg mL-1) and meropenem (18 μg mL-1). The results indicated a transient increase in clpB transcription in all treatments except cefepime. Both in the presence of ampicillin and amoxicillin/sulbactam for 20 min, the mRNA-clpB synthesis was 1.4 times higher than that of the control at time zero. Surprisingly, the mRNA-clpB levels were more than 30-fold higher after 10 min of incubation with meropenem and more than eightfold higher in the presence of trimethoprim/sulfamethoxazole. In addition, western blot assays showed that the RS4 strain treated with meropenem showed a marked increase in ClpB protein expression. Our data indicate that during exposure to beta-lactams, A. baumannii adjusts the transcription levels of the clpB mRNA and protein to respond to stress, suggesting that the chaperone may act as a key cellular component in the presence of antibiotics in this bacterium.