Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy

• DOI: 10.1007/s11033-020-05396-5
• Publication Date: 2020-04-17T18:04:11Z
• Authors: Farida Azzouz-Olden; Arthur G. Hunt; Randy Dinkins
• Keywords: Drought Tolerance; Candidate gene

Abstract Drought tolerance is a crucial trait for crops to curtail the yield loss inflicted by water stress, yet genetic improvement efforts are challenged complexity of this character. The adaptation sorghum abiotic its genotypic variability, and relatively small genome make species well-suited dissect molecular basis drought tolerance. use differential transcriptome analysis provides snapshot bioprocesses underlying response as well genes that might be determinants trait. RNA sequencing data were analyzed via gene ontology enrichment compare profiles two lines, drought-tolerant SC56 drought-sensitive Tx7000. outperformed Tx7000 in wet conditions upregulating processes driving growth guaranteeing homeostasis. seems an intrinsic occurring through overexpressing stress conditions, notably acting defense against oxidative (SOD1, SOD2, VTC1, MDAR1, MSRB2, ABC1K1). Similarly under drought, enhanced transmembrane transport maintained growth-promoting mechanisms. Under also upregulated heighten antioxidant capacity RCI3, VTE1, UCP1, FD1, FD2), regulatory factors (CIPK1 CRK7), repressors premature senescence (SAUL1). expression uncovered biological which upregulation enables better accumulator biomass connects key genes, making genotype judicious choice isolation genes.