Influence of shear stress and size on viability of endothelial cells exposed to gold nanoparticles

• DOI: 10.1007/s11051-017-3993-5
• Publication Date: 2017-09-11T18:30:25Z
• Authors: C. Fede; Giovanna Albertin; L. Petrelli; R. De Caro; I. Fortunati; V. Weber; Camilla Ferrante
• Keywords: Viability assay

Screening nanoparticle toxicity directly on cell culture can be a fast and cheap technique. Nevertheless, to obtain results in accordance with those observed live animals, the conditions which cells are cultivated should resemble one encountered systems. Microfluidic devices offer possibility satisfy this requirement, particular endothelial lines, because they capable reproduce flowing media shear stress experienced by these lines vivo. In work, we exploit microfluidic device observe how human umbilical vein (HUVEC) viability changes when subject continuous flow of medium, spherical citrate-stabilized gold nanoparticles different sizes at varying doses investigated. For comparison, same experiments also run multiwells where do not experience induced medium. We discuss considering influence mode exposure size (24 13 nm). that show lower under respect static HUVEC decreases as surface area per unit volume increases, regardless size.