Sustained Release of Multiple Growth Factors from Injectable Polymeric System as a Novel Therapeutic Approach Towards Angiogenesis

• DOI: 10.1007/s11095-009-0014-0
• Publication Date: 2009-11-30T22:17:18Z
• Authors: Qinghua Sun; Eduardo A Silva; Aixia Wang; James C. Fritton; David J. Mooney; Mitchell B. Schaf...; Paul M. Grossman; Sanjay Rajagopalan
• Keywords: Vascularity; Therapeutic angiogenesis; Hindlimb

The aim was to investigate that a bio-degradable alginate and poly lactide-co-glycolide (PLG) system capable of delivering growth factors sequentially would be superior single factor delivery in promoting neovascularization improving perfusion. Three groups apoE null mice underwent unilateral hindlimb ischemia surgery received ischemic limb intramuscular injections (Blank), containing VEGF165 (VEGF), or combined with PLG microspheres PDGF-BB (VEGF/PDGF). Vascularity the assessed by morphologic immunohistochemical end-points, while changes blood flow were Laser Doppler Perfusion Index. Muscle VEGF PDGF content at multiple time points. In VEGF/PDGF group, local tissue levels peaked week 2 4, respectively, detectable 6. At 6, mean vessel diameter significantly greater group compared Blank evidence well-formed smooth muscle-lined arterioles. Sequential using an injectable, biodegradable platform resulted stable sustained improvements This sustained, control-released, injectable polymer is promising approach for clinical application.