Structural insights into Arabidopsis ethylene response factor 96 with an extended N-terminal binding to GCC box

• DOI: 10.1007/s11103-020-01052-5
• Publication Date: 2020-08-19T11:02:41Z
• Authors: Chun-Yen Chen; Pei-Hsuan Lin; Kun-Hung Chen; Yi-Sheng Cheng
• Keywords: Helix-turn-helix; HMG-box; Sequence motif; Conserved sequence

Abstract The phytohormone ethylene is widely involved in many developmental processes and a crucial regulator of defense responses against biotic abiotic stresses plants. Ethylene-responsive element binding protein, member the APETALA2/ethylene response factor (AP2/ERF) superfamily, transcription that regulates stress-responsive genes by recognizing specific cis -acting target DNA. A previous study showed only NMR structure AP2/ERF domain AtERF100 complex with GCC box DNA motif. In this report, we determined crystal AtERF96 at atomic resolution. We analyzed residues conserved recognition sequence. addition to domain, an N-terminal α-helix participates interaction flanking region. also demonstrated EDLL motif, unique motif group IX family, might involve transactivation defense-related genes. Our establishes structural basis box, as well mechanisms domain.