Overexpression of Two Upstream Phospholipid Signaling Genes Improves Cold Stress Response and Hypoxia Tolerance, but Leads to Developmental Abnormalities in Barley

• DOI: 10.1007/s11105-019-01154-5
• Publication Date: 2019-07-22T18:02:28Z
• Authors: Krisztián Gierczik; András Székely; Mohamed Ahres; Zsuzsa Marozsán-Tóth; Ildikó Vashegyi; Wendy Harwood; Balázs Tóth; Gábor Galiba; Alexandra Soltész; Attila Vágújfalvi
• Keywords: 0301 basic medicine; 03 medical and health sciences; SB Plant culture / növénytermesztés; 3. Good health

Phosphatidylinositol transfer protein (PITP) and phosphatidylinositol 4-kinase (PI4K) are very upstream regulatory elements of the phospholipid signaling pathway in signal transduction network. Unlike animal systems, their role stress is poorly understood plants. To study this area, PITP- PI4K-overexpressing transgenic barley lines were developed. Morphological developmental abnormalities surveyed characterized. It was revealed that overexpression genes led to more phenotypic than other studies working with effector or even transcription factors. We hypothesize high level consequence modulation elements. On hand, we also PITP PI4K increased tolerance during hypoxic cold stress, but not salinity stress. Differences found frost between plants recipient Golden Promise line. Molecular analysis showed improvement related most important responsive factors, CBF genes. conclude method may be useful prove an element; however, due many consequences occur as side effects, it a less advisable approach achieve improved tolerance.