Early generation in vitro assay to identify potato populations and clones tolerant to heat

• DOI: 10.1007/s11240-014-0677-z
• Publication Date: 2014-12-08T09:04:18Z
• Authors: M. Awais Khan; Susan Munive; Merideth Bonierbale
• Keywords: Growing season

An efficient in vitro system for early generation selection of heat-tolerant potato breeding materials was tested and validated field conditions. At the family level, groups expected to be heat tolerant due their genetic background were identified as tolerant. In assay, LTVR × LTVR, an advanced population developed at CIP, had 100 % plants with tubers 18 °C, 73 25 °C 2 32 dark. The results from true seed level screening tuber evaluation under conditions Tacna, arid sub-tropical environment Southern Peru, positively correlated (r = 0.57). There low moderate correlation between percentage 27 temperature treatment harvest index vivo Majes–Arequipa, San Ramon, La Molina that followed increasing ranges sites. This indicates methodology can predict putative clones a confidence. Low is possibly differential responses characteristics growing environment, such soil versus media, which not represented well fact field, day–night temperatures vary during tuberization filling, throughout season, while dark period kept constant, controlled specifically assess (tuber induction) high night temperatures. ability seedling assay identify families inexpensive less time consuming way without need transplanting experimental material will facilitate significant samples resources improved populations programs attempting improve adaptation new environments climate change.