Blackberry synthetic seeds storage: effects of temperature, time, and sowing substrate

• DOI: 10.1007/s11240-024-02816-3
• Publication Date: 2024-07-03T17:02:00Z
• Authors: Luca Regni; Maurizio Micheli; Alberto Marco Del...; Simona Lucia Facchin; Emanuele Rabica; Leonardo Camilloni; Arianna Cesarini; Primo Proietti
• Keywords: Potting; Perlite; Plantlet; Germ plasm

Abstract In vitro propagation, is becoming the predominant method for blackberry propagation due to its advantages compared agamic traditional methods. Synthetic seed technology represents a promising approach further enhance productivity of in facilitating exchange plant materials among laboratories and contributing germplasm conservation efforts. This study aimed establish an optimal protocol storage sowing synthetic seeds obtained through encapsulation clump bases. The were sown without (Control) after periods 30, 60, 120 days at 4 °C 25 dark, three different substrates (agarised, perlite, potting). After forty-five from viability, regeneration rate, shoot root numbers lengths, as well fresh dry weights plantlets, assessed. Results indicated that agarised substrate consistently exhibited favourable outcomes, with sustained rates robust plantlet development even prolonged °C. perlite potting demonstrated enhanced following 60 days. On contrary, resulted notable decline highlighting inadequacy purposes. These findings underscore importance selection temperature optimizing protocols seeds.