Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor

• DOI: 10.1007/s11248-015-9873-9
• Publication Date: 2015-03-29T06:22:30Z
• Authors: Aleksandar Cingel; Jelena Savić; Branka Vinterhalter; Dragan Vinterhalter; Miroslav Kostić; Darka Šešlija Jov...; Ann Smigocki; Slavica Ninković
• Keywords: 0301 basic medicine; 2. Zero hunger; Oryzacystatin II; Plants, Genetically Modified; Real-Time Polymerase Chain Reaction; Pest management; Cystatins; 630; Gene Expression Regulation, Enzymologic; Coleoptera; Plant Leaves; 03 medical and health sciences; Gene Expression Regulation, Plant; Larva; Colorado potato beetle; Animals; Protease Inhibitors; Proteinase inhibitor; Pest Control; Disease Resistance; Solanum tuberosum

Plant proteinase inhibitors (PIs) are attractive tools for crop improvement and their heterologous expression can enhance insect resistance in transgenic plants. PI oryzacystatin II (OCII), isolated from rice, showed potential in controlling pests that utilize cysteine proteinases for protein digestion. To evaluate the applicability of the OCII gene in enhancing plant defence, OCII-transformed potatoes were bioassayed for resistance to Colorado potato beetle (Leptinotarsa decemlineata Say). Feeding on transformed leaves of potato cultivars Desiree and Jelica significantly affected larval growth and development, but did not change mortality rates. During the L2 and L3 developmental stages larvae consumed the OCII-transformed foliage faster as compared to the nontransformed control. Also these larvae reached the prepupal stage (end of L4 stage) 2 days earlier than those fed on control leaves. However, the total amounts of consumed OCII-transformed leaves were up to 23% lower than of control, and the maximal weights of prepupal larvae were reduced by up to 18% as compared to larvae fed on nontransformed leaves. The reduction in insect fitness reported in this study in combination with other control measures, could lead to improved CPB resistance management in potato.