Molecular characterization of African swine fever virus from domestic pigs in northern Tanzania during an outbreak in 2013

• DOI: 10.1007/s11250-014-0628-z
• Publication Date: 2014-07-04T05:23:15Z
• Authors: Gerald Misinzo; David E. Kwavi; Christopher D. Si...; Mariam Makange; Emma Peter; Amandus P. Muhairwa; Michael J. Madege
• Keywords: 0303 health sciences; Base Sequence; Genotype; Swine; Molecular Sequence Data; Sus scrofa; Sequence Analysis, DNA; African Swine Fever Virus; Polymerase Chain Reaction; Tanzania; 3. Good health; Viscera; 03 medical and health sciences; Food Animals; Animals; Cluster Analysis; Animal Science and Zoology; Capsid Proteins; African Swine Fever; Phylogeny; Regular Articles; DNA Primers

African swine fever (ASF) is an acute, highly contagious and deadly viral hemorrhagic of domestic pigs caused by virus (ASFV), a double-stranded DNA the family Asfarviridae. In this study, molecular diagnosis characterization outbreak ASFV in northern Tanzania, was performed on spleen, lymph node, kidney, heart samples collected June July 2013 from that died during disease outbreak. Confirmatory ASF using polymerase chain reaction (PCR) partial amplification B646L gene encoding major capsid protein p72 PPA1/PPA2 primers. PCR primers produced expected product size, confirming Tanzania. addition, nucleotide sequencing, phylogenetic reconstruction variable 3′-end complete E183L inner envelope transmembrane p54 showed Tanzania were 100 % identical clustered into (p72) (p54) genotype X. Furthermore, tetrameric amino acid repeats within central region (CVR) B602L coding for J9L had signature BNBA(BN)5NA with single novel tetramer NVDI (repeat code N). The results present study confirm year show closely related to other ticks, warthogs, previously reported