Regulatory effect of Balanites aegyptiaca ethanol extract on oxidant/antioxidant status, inflammatory cytokines, and cell apoptosis gene expression in goat abomasum experimentally infected with Haemonchus Contortus

• DOI: 10.1007/s11250-024-04023-w
• Publication Date: 2024-07-04T05:01:25Z
• Authors: Doaa Sedky; Tamer Helmi Abd E...; Soad Mohamed Nasr; Sekena Hassanien ...; Noha Mahmoud Fahm...; Amira Hassan Mohamed; Hala Abdalla Ahme...
• Keywords: Balanites aegyptiaca

Abstract This experiment aimed to assess the regulatory effects of treatment with Balanites aegyptiaca fruit ethanol extract (BA-EE) on oxidant/antioxidant status, anti-inflammatory cytokines, and cell apoptosis gene expression in abomasum Haemonchus contortus –infected goats. Twenty goat kids were assigned randomly four equal groups: (G1) infected-untreated, (G2) uninfected-BA-EE-treated, (G3) infected-albendazole-treated, (G4) infected-BA-EE-treated. Each (G1), (G3), was orally infected 10,000 infective third-stage larvae. In fifth week postinfection, single doses albendazole (5 mg/kg.BW) BA-EE (9 g/kg.BW) given orally. ninth animals slaughtered obtain specimens. The following markers determined: malondialdehyde (MDA), glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD), catalase (CAT). mRNA cytokines (IL-3, IL-6, IL-10, TNF-α) (Bax, Bcl-2) estimated. showed significantly reduced GSH content GST SOD activities but a markedly increased MDA level. revealed lower level pronouncedly elevated GSH, SOD, levels. antioxidant properties superior those albendazole. expressions IL-3, TNF-α, Bax-2 upregulated downregulated (G4). Bcl-2 Bcl-2/Bax ratio followed reverse course both treated groups. We conclude that has protective role H. could be attributed its ability reduce pro-inflammatory apoptosis.