Interaction between Translocation-associated membrane protein 1 and σC protein of novel duck reovirus controls virus infectivity

• DOI: 10.1007/s11262-020-01750-8
• Publication Date: 2020-03-16T17:03:51Z
• Authors: Rong Xiao; Xiaoyun Mi; Jiahui Sun; Mingyang Ding; Chuanfeng Li; Jie Zhu; Guangqing Liu; Wenge Ma; Hailong Zhou; Zongyan Chen
• Keywords: 0301 basic medicine; Membrane Glycoproteins; Orthoreovirus, Avian; Fluorescent Antibody Technique; Virus Replication; Reoviridae Infections; 3. Good health; Viral Proteins; 03 medical and health sciences; Ducks; Host-Pathogen Interactions; Animals; RNA, Small Interfering; Protein Binding

Novel duck reovirus (NDRV), the prototype strain of the species Avian orthoreovirus (ARV), is associated with high mortality in Pekin ducklings. σC is an outer capsid protein encoded by the S1 genome segment of NDRV which mediates attachment to host cells. Our previous studies using immunoprecipitation and mass spectrometry found that σC coprecipitated with some host proteins including Translocation-associated membrane protein 1 (TRAM1). However, the interaction between σC and TRAM1 has not been further confirmed experimentally. In this study, we utilized coimmunoprecipitation assays, glutathione S-transferase pull-down, and confocal microscopy to confirm the interaction between σC and TRAM1. In addition, knockdown of TRAM1 using siRNA and overexpression of TRAM1 gene were conducted to explore its effect on virus replication. The result showed that TRAM1 silencing benefits while overexpression inhibits viral replication. This study confirms the important role TRAM1 during NDRV infection which can help develop new approaches for NDRV disease prevention and control.