Temporal landscape of mutational frequencies in SARS-CoV-2 genomes of Bangladesh: possible implications from the ongoing outbreak in Bangladesh

• DOI: 10.1007/s11262-021-01860-x
• Publication Date: 2021-07-12T14:02:41Z
• Authors: Otun Saha; Israt Islam; Rokaiya Nurani Sh...; Nadira Naznin Rakhi; Md. Shahadat Hossain; Md. Mizanur Rahaman
• Keywords: Mega-; Mutation Accumulation

Along with intrinsic evolution, adaptation to selective pressure in new environments might have resulted the circulatory SARS-CoV-2 strains response geoenvironmental conditions of a country and demographic profile its population. With this target, current study traced evolutionary route mutational frequency 198 Bangladesh-originated genomic sequences available GISAID platform over period 13 weeks as 14 July 2020. The analyses were performed using MEGA X, Swiss Model Repository, Virus Pathogen Resource Jalview visualization. Our analysis identified that majority circulating strikingly differ from both reference genome first sequenced Bangladesh. Mutations nonspecific proteins (NSP2-3, NSP-12(RdRp), NSP-13(Helicase)), S-Spike, ORF3a, N-Nucleocapsid protein common varying degrees most unique mutations (UM) found NSP3 (UM-18). But no or limited changes observed NSP9, NSP11, Envelope (E) accessory factors (NSP7a, ORF 6, ORF7b) suggesting possible conserved functions those propagation. However, along D614G mutation, more than 20 different Spike detected basically S2 domain. Besides, SR-rich region N P323L RDRP also present. mutation accumulation showed significant association (p = 0.003) sex age COVID-19-positive cases. So, identification these patterns may greatly facilitate vaccine development deciphering sex-dependent differential susceptibility COVID-19.