Differential Tolerance to Lead and Cadmium of Micropropagated Gypsophila fastigiata Ecotype

• DOI: 10.1007/s11270-018-3702-8
• Publication Date: 2018-01-25T16:27:48Z
• Authors: Ewa Muszyńska; Ewa Hanus-Fajerska; Aleksandra Koźmińska
• Keywords: Ecotype

In vitro techniques may provide a suitable tool for effective propagation and conservation of plant species representing various ecological niches. The elaboration such protocols is also prerequisite selection heavy-metal-tolerant material that could be afterwards used restoration or remediation polluted sites. this study, culture protocol Gypsophila fastigiata was developed. highest multiplication coefficient, which reached 6.5, the best growth parameters were obtained on modified MS medium supplemented with 1.0 mg L−1 2iP 0.2 IAA. cultures treated different concentrations lead nitrate (0.1, 0.5, mM Pb(NO3)2) cadmium chloride (0.5, 2.5, 5.0 μM CdCl2). parameters, photosynthetic pigments, phenolic compound content examined in order to evaluate whether tested metal salts can have an adverse impact studied culture. It ascertained Pb ions induced disturbances contributed shoot wither. On contrary, proliferative established media containing Cd coefficients length increased all enriched CdCl2. Chlorophylls carotenoid contents negatively affected by application cadmium; nevertheless, shoots 2.5 CdCl2, accumulation pigments occurred their amount similar untreated Adaptation associated stimulation synthesis. Hence, we reported unambiguous positive result procedure obtain vigorous tolerant cadmium.