Evaluation of different bacterial honey isolates as probiotics and their efficient roles in cholesterol reduction

• DOI: 10.1007/s11274-022-03259-8
• Publication Date: 2022-05-04T05:08:35Z
• Authors: Noura O. Abdelsamad; Mona A. Esawy; Zeinab E. Mahmoud; Asmaa I. El-Shazly; Tarek R. Elsayed; Amira A. Gamal
• Keywords: Staphylococcus aureus; Pharmacology, Toxicology and Pharmaceutics; Materials Science; Immunology; Microbiology; Biochemistry; Food science; 03 medical and health sciences; Antibiotics; RNA, Ribosomal, 16S; Materials Chemistry; Genetics; Biology; Gentamicin; Pharmacology; Immunology and Microbiology; 2. Zero hunger; Original Paper; 0303 health sciences; Macromolecular Crystallography Techniques; Bacteria; Protein Stability; Probiotics; FOS: Clinical medicine; Life Sciences; Honey; Hydrogen Peroxide; 3. Good health; Immunotoxin Therapy for Cancer Treatment; Cholesterol; Pharmacological Effects of Licorice Roots; FOS: Biological sciences; Physical Sciences; Incubation; Bacillus subtilis

Continue to hypothesize that honey is a storehouse of beneficial bacteria, and the majority these isolates are levansucrase producers. Accordingly, ten bacterial strains were isolated from different sources. Four had highest production levan yield identified by partial sequencing 16S rRNA gene as Achromobacter sp. (10A), Bacillus paralicheniformis (2M), subtilis (9A), paranthracis (13M). The cytotoxicity selected showed negative blood hemolysis. Also, they sensitive tested antibiotics (Amoxicillin + Flucloxacillin, Ampicillin, Gentamicin, Benzathine benzylpenicillin, Epicephin, Vancomycin, Amikacin, Zinol). strong alkaline stability (pHs 9, 11) resistant severe acidic conditions (29-100 percent). recorded complete tolerance both H2O2 bile salt (0.3% Oxgall powder) after 24 h incubation. cell-free supernatant examined antifungal activities against C. Albicans with varying degrees. 2M 13M S. aureus. adhesion auto-aggregation capacity. Isolate 10A antioxidant activity (91.45%) followed (47.37%). catalase protease activity. All produced cholesterol oxidase lipase levels. Besides, four reduced LDL (low-density lipoprotein) significant values. cholesterol-reducing ability varied not only for but also time previous results recommended be used safely in solving problem.