Complete plastid genome sequence of Vaccinium macrocarpon: structure, gene content, and rearrangements revealed by next generation sequencing

• DOI: 10.1007/s11295-012-0573-9
• Publication Date: 2012-11-06T13:08:02Z
• Authors: Diego Fajardo; Douglas Senalik; Mercedes Ames; Huayu Zhu; Shawn A. Steffan; Rebecca Harbut; James Polashock; Nicholi Vorsa; Emily Gillespie; Kathy Kron; Juan E. Zalapa
• Keywords: 0303 health sciences; 03 medical and health sciences

The complete plastid genome sequence of the American cranberry (Vaccinium macrocarpon Ait.) was reconstructed using next-generation sequencing data by in silico procedures. We used Roche 454 shotgun sequence data to isolate cranberry plastid-specific sequences of"HyRed" via homologycomparisonswithcompletesequencesfromseveral species available at the National Center for Biotechnology Information database. Eleven cranberry plastid contigs were selected for the construction of the plastid genome-based homologies and on raw reads flowing through contigs and connection information. We assembled and annotated a cran- berry plastid genome (82,284 reads; 185x coverage) with a length of 176 kb and the typical structure found in plants, but with several structural rearrangements in the large single-copy region when compared to other plastid asterid genomes. To evaluate the reliability of the sequence data, phylogenetic analysis of 30 species outside the order Ericales (with 54 genes) showed Vaccinium inside the clade Asteridae ,a s reported in other studies using single genes. The cranberry plastid genome sequence will allow the accumulation of crit- ical data useful for breeding and a suite of other genetic studies.