Metabolomic profile of diet-induced obesity mice in response to humanin and small humanin-like peptide 2 treatment

• DOI: 10.1007/s11306-019-1549-7
• Publication Date: 2019-06-06T17:02:27Z
• Authors: Hemal H. Mehta; Jialin Xiao; Ricardo Ramirez; Brendan Miller; Su-Jeong Kim; Pinchas Cohen; Kelvin Yen
• Keywords: Sphingolipid

The mitochondrial-derived peptides (MDPs) are a novel group of natural occurring that have important signaling functions and biological activity. Both humanin small-humanin-like peptide 2 (SHLP2) been reported to act as insulin sensitizers modulate metabolism. By using metabolomic approach, this study explores how the plasma metabolite profile is regulated in response SHLP2 treatment diet-induced obesity (DIO) mouse model. results also shed light on potential mechanism underlying MDPs' sensitization effects. Plasma samples were obtained from DIO mice subjected vehicle (water) treatment, or with either analog S14G (HNG) (n = 6 per group). Vehicle given intraperitoneal (IP) injections twice day at dose 2.5 mg/kg/injection for 3 days. Metabolites comprehensively identified quantified UPLC-MS/MS. HNG administration significantly altered concentrations amino acid lipid metabolites plasma. Among all metabolic pathways, glutathione sphingolipid metabolism responded most strongly treatment. present indicates can lower several markers associated age-related disorders. With previous understanding effects cardiovascular function, sensitization, anti-inflammation, discovery provides more comprehensive molecular explanation action