Irradiation, Cisplatin, and 5-Azacytidine Upregulate Cytomegalovirus Promoter in Tumors and Muscles: Implementation of Non-invasive Fluorescence Imaging

• DOI: 10.1007/s11307-010-0300-6
• Publication Date: 2010-04-15T15:35:43Z
• Authors: Urska Kamensek; Gregor Sersa; Suzana Vidic; Gregor Tevz; Simona Kranjc; Maja Cemazar
• Keywords: green fluorescent protein; 0301 basic medicine; Fibrosarcoma; Cytomegalovirus; animal cell; Inbred C57BL; Fluorescence imaging; Mice; CMV promoter; Genes, Reporter; breast carcinoma; Promoter Regions, Genetic; Inbred BALB C; Medicine(all); Microscopy; Mice, Inbred BALB C; DNA methylation; Tumor; Muscles; Mus; article; Up-Regulation; 3. Good health; female; priority journal; Mammalia; Azacitidine; Female; Research Article; azacitidine; in vitro study; Infrared Rays; animal experiment; Immediate-Early; Fluorescence; Cell Line; Promoter Regions; in vivo study; 03 medical and health sciences; drug activity; promoter region; Genetic; plasmid; Cell Line, Tumor; In vivo; Animals; controlled study; Reporter; Genes, Immediate-Early; mouse; nonhuman; animal model; DNA Methylation; Demethylation; Mice, Inbred C57BL; leg muscle; Genes; Mammary carcinoma; Microscopy, Fluorescence; gene expression; Irradiation; Murinae; genetic transfection; Cisplatin

The cytomegalovirus (CMV) promoter is one of the most commonly used promoters for expression transgenes in mammalian cells. aim our study was to evaluate role methylation and upregulation CMV by irradiation chemotherapeutic agent cisplatin vivo using non-invasive fluorescence imaging.Murine fibrosarcoma LPB mammary carcinoma TS/A cells were stably transfected with plasmids encoding p21 promoter-driven green fluorescent protein (GFP) gene. Solid tumors induced subcutaneous injection tumor cells, while leg muscles transiently plasmid GFP under control promoter. Cells, tumors, legs treated either DNA inhibitor 5-azacytidine, irradiation, or cisplatin. determined a microplate reader vitro imaging vivo.Treatment 5-azacytidine (re)activated Furthermore, treatment resulted significant both vivo.Observed alterations activity limit usefulness this widely as constitutive On other hand, inducibility can be beneficially gene therapy when combined standard cancer treatment, such radiotherapy chemotherapy.