Characterization of hydrocarbon-degrading and biosurfactant-producing Pseudomonas sp. P-1 strain as a potential tool for bioremediation of petroleum-contaminated soil

• DOI: 10.1007/s11356-014-2872-1
• Publication Date: 2014-04-17T05:20:28Z
• Authors: Magdalena Pacwa-P...; Grażyna Anna Płaza; Anna Poliwoda; Zofia Piotrowska-...
• Keywords: Rhamnolipid; Strain (injury); Enrichment culture; Soil microbiology

The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. strain degraded crude oil, fractions A5 P3 of hexadecane (27, 39, 27 13% added culture medium were degraded, respectively) but had no ability phenanthrene. Additionally, the presence gene-encoding enzymes responsible degradation alkanes naphthalene in genome reported. Positive results blood agar methylene blue tests, as well gene rhl, involved biosynthesis rhamnolipid, confirmed synthesis glycolipid 1H 13C nuclear magnetic resonance, Fourier transform infrared spectrum mass analyses indicated that extracted biosurfactant affiliated with rhamnolipid. this study indicate and/or produced by have potential be used bioremediation soils.