Metal toxicokinetics and metal-driven damage to the gut of the ground beetle Pterostichus oblongopunctatus

• DOI: 10.1007/s11356-016-7412-8
• Publication Date: 2016-08-19T05:05:49Z
• Authors: Agnieszka J. Bedn...; Ryszard Laskowski; Elżbieta Pyza; Danuta Semik; Zuzanna Świątek; Olga Woźnicka
• Keywords: Male; 0301 basic medicine; cadmium; Health, Toxicology and Mutagenesis; Apoptosis; Models, Biological; nickel; 03 medical and health sciences; Microscopy, Electron, Transmission; Nickel; In Situ Nick-End Labeling; Environmental Chemistry; Animals; insects; intestine; zinc; invertebrates; trace metal; Pollution; Toxicokinetics; Coleoptera; Gastrointestinal Tract; Zinc; physiology; cells; Environmental Pollutants; Research Article; Cadmium

Toxicokinetics makes up the background for predicting concentrations of chemicals in organisms and, thus, ecological risk assessment. However, physiological and toxicological mechanisms behind toxicokinetics particular are purely understood. The commonly used one-compartment model has been challenged recently, showing that case metals it does not describe pattern observed terrestrial invertebrates exposed to highly contaminated food. We hypothesised main mechanism shaping at high exposure food is cellular damage gut epithelial cells. Gut should result decreased metal assimilation rate, while shedding dead cells - increased elimination rate. performed a typical toxicokinetic experiment, feeding ground beetles Pterostichus oblongopunctatus with Cd, Ni or Zn 40 mM kg-1 28 days, followed by depuration period 14 days on uncontaminated male were sampled throughout experiment body histopathological examinations midgut. All exhibited complex internal over time, an initial rapid increase decrease fluctuating during further exposure. Histopathological studies showed massive midgut epithelium, marked differences between metals. Cd appeared most toxic caused immediate cell degeneration. effects more gradual pronounced after least 1 week also extensive degeneration epithelium but its weakest among studied