Genotoxic potential of diesel exhaust particles from the combustion of first- and second-generation biodiesel fuels—the FuelHealth project

• DOI: 10.1007/s11356-017-9995-0
• Publication Date: 2017-09-11T15:06:55Z
• Authors: Magdalena Kowalska; Aneta Wegierek-Ciuk; Kamil Brzoska; Maria Wojewodzka; Sylwia Meczynska-...; Joanna Gromadzka-...; Remigiusz Mruk; Johan Øvrevik; Marcin Kruszewski; Anna Lankoff
• Keywords: Comet Assay

Epidemiological data indicate that exposure to diesel exhaust particles (DEPs) from traffic emissions is associated with higher risk of morbidity and mortality related cardiovascular pulmonary diseases, accelerated progression atherosclerotic plaques, possible lung cancer. While the impact DEPs combustion fossil fuel on human health has been extensively studied, current knowledge biofuels provides limited inconsistent information about its mutagenicity genotoxicity, as well adverse risks. The objective present work was compare genotoxicity two first-generation fuels, 7% fatty acid methyl esters (FAME) (B7) 20% FAME (B20), a second-generation FAME/hydrotreated vegetable oil (SHB: synthetic hydrocarbon biofuel) fuel. Our results revealed particulate engine each type biodiesel induced genotoxic effects in BEAS-2B A549 cells, manifested increased levels single-strand breaks, frequencies micronuclei, or deregulated expression genes involved DNA damage signaling pathways. We also found none tested showed induction oxidative gamma-H2AX-detectable double-strand breaks. most pronounced differences concerning were observed for greatest being B7-derived DEPs. other between different blend percentage feedstock observed, but magnitude these variations limited.