Alone and combined toxicity of ZnO nanoparticles and graphene quantum dots on microalgae Gymnodinium

• DOI: 10.1007/s11356-022-19267-y
• Publication Date: 2022-02-18T00:03:35Z
• Authors: Xiaolin Zhu; Liju Tan; Ting Zhao; Wenqiu Huang; Xin Guo; Jiayin Wang; Jiangtao Wang
• Keywords: Quantum Dots; Dinoflagellida; Microalgae; 0211 other engineering and technologies; Nanoparticles; Graphite; 02 engineering and technology; Zinc Oxide

Investigation of ZnO nanoparticles (nano-ZnO) and graphene quantum dots (GQDs) toxicology on dinoflagellate Gymnodinium helps to understand the effects of different surface characteristic nanoparticles on marine algae. The growth and biological responses of the algae exposed to 1, 10, 20 mg L-1 nano-ZnO and GQDs in f/2 media were explored. Nano-ZnO showed slight effects on algal cells growth, while the growth inhibition rates of Gymnodinium increased as GQDs concentration increasing. Both nanoparticle treatments induced accumulation of reactive oxygen species and activated intracellular antioxidant defensive system, including SOD and ATPase, which were related to the two nanoparticles concentration. Under combined exposure of nano-ZnO and GQDs, the inhibitory effects decreased compared to the single GQDs and showed antagonistic effect. The addition of nano-ZnO could decrease the toxicity of GQDs due to aggregation and sedimentation interaction between nanoparticles. The morphologic change of the cells observed by SEM proved that nanoparticles adsorbed onto the cell surfaces and caused the cell shrinkage.