Pyroptosis participates in PM2.5-induced air–blood barrier dysfunction

• DOI: 10.1007/s11356-022-20098-0
• Publication Date: 2022-04-18T12:03:14Z
• Authors: Ruxue Jia; Min Wei; Xiao Zhang; Rui Du; Wenping Sun; Lili Wang; Laiyu Song
• Keywords: 0301 basic medicine; Mice; 03 medical and health sciences; Blood-Air Barrier; Inflammasomes; Pyroptosis; Animals; Particulate Matter; Lung Injury; Reactive Oxygen Species

Epidemiological studies have shown that particulate matters with diameter less than 2.5 μm (PM2.5) play an important role in inducing and promoting respiratory diseases, but its underlying mechanism remains to be explored. The air-blood barrier, also known as the alveolar-capillary barrier, is the key element of the lung, working as the site of oxygen and carbon dioxide exchange between pulmonary vasculatures. In this study, a mouse PM2.5 exposure model was established, which leads to an induced lung injury and air-blood barrier disruption. Oxidative stress and pyroptosis were observed in this process. After reducing the oxidative stress by N-acetyl-L-cysteine (NAC) treatment, the air-blood barrier function was improved and the effect of PM2.5 was alleviated. The level of pyroptosis and related pathway were also effectively relieved. These results indicate that acute PM2.5 exposure can cause lung injury and the alveolar-capillary barrier disruption by inducing reactive oxygen species (ROS) with the participation of pyroptosis pathway.